Fig. 3

BWSTT inhibits neuronal cell death and promotes axonal regeneration after SCI. A Representative immunofluorescence images of NeuN (a neuronal marker) (green) from the anterior horn area of the spinal cord (transverse sections of the caudal region of the injured spinal cord) in each group following SCI (n = 3). Nuclei were counterstained with DAPI (blue). Scale bar = 100 μm, 50 μm. B, C Quantification of the number of NeuN+ cells/mm2 in (A) and NF200+ fluorescence intensity area in (D). D Representative immunofluorescence images of NF200 (key components of the neuronal cytoskeleton) (green) and DAPI (blue) in longitudinal sections of the spinal cord from each group after SCI (n = 3). Scale bar = 500 μm and * Indicates lesion center. Values are expressed as the mean ± s.e.m, * p < 0.05, ** p < 0.01, and *** p < 0.001, as determined by one-way ANOVA