Fig. 3

VMNdm versus VMNvl GABAergic Nerve Cell Co-Expressed Counterregulatory Neurochemical Marker mRNA Content: Effects of VMN nNOS Gene Silencing. Box-and-whisker plots depict normalized values for GABAergic nerve cell mRNAs encoding, growth hormone-releasing hormone (Ghrh), or the vesicular glutamate transporter-1 (Slc1A2), a marker for glutamate. Plots depict normalized Ghrh [Fig. 3A (VMNdm) and 3B (VMNvl)], or Slc1A2 [Fig. 3C (VMNdm) and 3D (VMNvl)] values derived from each VMN GABA nerve cell subpopulation neurons for the following treatment groups: SCR siRNA/sc V (blue box-and-whisker plots, n = 12), nNOS siRNA/sc V (yellow box-and-whisker plots; n = 12), SCR siRNA/sc INS (green box-and-whisker plots; n = 12), or nNOS siRNA/sc INS (orange box-and-whisker plots; n = 12). Statistical differences between discrete pairs of treatment groups are indicated by *p < 0.05; **p < 0.01; or ***p < 0.001