Fig. 2

LPS-treated LRRK2 G2019S KI mice exhibit slower weight recovery and increased LRRK2 autophosphorylation at Ser1292. (A) Mice of the indicated genotypes were treated with a single dose of LPS (5 mg/kg, i.p.). Body weights were monitored daily for two weeks. The percentages of initial body weights are shown. The number of mice: WT, n = 8; LRRK2 G2019S KI, n = 11. One WT mouse died on day four post-LPS injection and was excluded from the study. Striatal extracts from naive WT and LRRK2 G2019S-KI mice (B) and saline- or LPS-treated mice (C, D) were subjected to Western blot analysis. Representative blots of LRRK2, P-LRRK2 (Ser1292, Panel C), and P-Rab12 (T72, Panel D) and corresponding quantification are shown (below). n = 5–8 mice per group. For (B), ^**P < 0.01; two-tailed Student’s t test; for (C), ^**P < 0.01; ****P < 0.0001; one-way ANOVA followed by Tukey’s multiple comparison post hoc test. The data are presented as the means ± SEMs