Fig. 1

LPS administration does not cause dopaminergic neuron loss in WT or LRRK2 G2019S-KI mice within two weeks. (A) Striatal dopamine (left) and DOPAC (right) levels in WT and LRRK2 G2019S-KI mice injected with saline or LPS were analyzed by HPLC-ECD. (B) Representative images of brain sections containing TH⁺ neurons in the substantia nigra regions (left) and quantification of TH⁺ neurons in the substantia nigra area (right). Scale bar, 500 μm. (C) Striatal TH expression levels were measured by Western blot analysis (left). Densitometric quantifications are shown (right). α-Tubulin served as a loading control. n = 4 mice per group. For (A − C), one-way ANOVA followed by Tukey’s multiple comparison post hoc test was used. The data are presented as the means ± standard errors of the means (SEMs)