Table 5 Stability values and rankings according to BestKeeper, geNorm, NormFinder, DeltaCt, and RefFinder
Gene | BestKeeper | geNorm | NormFinder (without group identifiers) | NormFinder (with group identifiers) | DeltaCt | Comprehensive ranking (RefFinder) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
MAD | Rank | M value | Rank | S value | Rank | S value | Rank | SDavg | Rank | GMR | Rank | |
Actb | 1.034 | 5 | 0.529 | 5 | 0.580 | 5 | 0.306 | 4 | 0.639 | 5 | 5.000 | 5 |
Gapdh | 0.762 | 4 | 0.456 | 4 | 0.159 | 1 | 0.125 | 1 | 0.451 | 1 | 2.000 | 2 |
B2m | 0.452 | 2 | 0.293 | 1 | 0.202 | 2 | 0.157 | 2 | 0.451 | 2 | 1.682 | 1 |
Rpl13a | 0.413 | 1 | 0.293 | 1 | 0.494 | 4 | 0.309 | 5 | 0.560 | 4 | 2.000 | 2 |
Hprt | 0.503 | 3 | 0.423 | 3 | 0.355 | 3 | 0.282 | 3 | 0.527 | 3 | 3.000 | 4 |