Skip to main content

Table 3 Ct descriptive statistics obtained by BestKeeper

From: Evaluation of suitable reference genes for gene expression studies in the developing mouse cortex using RT-qPCR

 

Actin

Gapdh

B2m

Rpl13a

Hprt

Arithmetic mean (Ct)

18.99

20.58

26.01

19.58

25.07

Geometric mean (Ct)

18.96

20.57

26.00

19.57

25.06

Min (Ct)

17.22

19.39

25.16

18.69

24.09

Max (Ct)

20.76

21.93

27.09

20.68

26.02

MAD (± Ct)

1.034

0.762

0.452

0.413

0.503

CV (% Ct)

5.44

3.70

1.74

2.11

2.01

Min (x-fold)

-3.34

-2.26

-1.79

-1.84

-1.96

Max (x-fold)

3.49

2.58

2.13

2.16

1.95

MAD (± x-fold)

2.05

1.70

1.37

1.33

1.42

n

18

18

18

18

18

  1. Min, minimum; Max, maximum; MAD, average absolute deviation; CV, coefficient of variance; n, sample size