Evaluation of suitable reference genes for gene expression studies in the developing mouse cortex using RT-qPCR

BMC Neuroscience

Table 1 List of candidate reference genes and primer characteristics

Gene symbol	Gene name	Function	UniGene ID	Assay ID	Assay design	Amplicon size (bp)	Amplification efficiency (%)
Actb	β-actin	Cytoskeletal structural protein	Mm.391967	qMmuCED0027505	Exonic	109	101
Gapdh	Glyceraldehyde-3-phosphate dehydrogenase	Glycolysis enzyme	Mm.304088	qMmuCED0027497	Exonic	75	101
B2m	β2 microglobulin	Component of major histocompatibility complex (MHC) class I molecules	Mm.163	qMmuCID0040553	Intron-spanning	112	100
Rpl13a	Ribosomal protein L13A	Ribosome component; protein synthesis	Mm.180458	qMmuCED0040629	Exonic	70	90
Hprt	Hypoxanthine-guanine phosphoribosyl transferase	Purine synthesis in salvage pathways	Mm.299381	qMmuCID0005679	Intron-spanning	70	94

ISSN: 1471-2202