Fig. 1
From: Neural progenitor cell-derived exosomes in ischemia/reperfusion injury in cardiomyoblasts
Complete Workflow. Supernatant was collected at multiple time points from 1 neurospheres, 2 adherent neural stem cell cultures, and 3 from cells grown in CL 1000 bioreactor, for serial ultracentrifugation steps. 20k and 110k harvests were resuspended and frozen for further analysis. H9c2 cardiomyoblasts were cultured in FX seahorse plates, subjected to 4-hour and 6-hour oxygen glucose deprivation, followed by treatment with exosomes. Supernatant was collected for LDH assay, and mitochondrial respiration was measured using FX seahorse