Fig. 1

Psilocybin (Psi) dose-dependently reduced glutamate-mediated neuronal loss in rat primary cortical neuronal cultures. (A) Representing photomicrographs indicate that glutamate (Glu, 100µM) reduced MAP2 immunoreactivity (MAP2-ir). Glu-mediated neuronal loss was antagonized by psilocybin (Psi, 10µM). (B) MAP2-ir from all studies was averaged and analyzed. Glu significantly reduced MAP2-ir (p<0.001, F_4,35=42.103, one-way ANOVA+NK test). Psi dose-dependently mitigated this degenerative response (*p=0.010, **p=0.011, **p=0.003, number of wells=6–12 per group, F_4,35=42.103, one-way ANOVA+NK test). (C) Timeline. +AO: with antioxidant. -AO: without antioxidant. Calibration: 100 µm