Fig. 8

Illustrates the labeling of nematode terminal pharyngeal bulbs using mitochondria-targeted dyes (MitoSOX) to measure superoxide levels. MitoSOX fluorescence demonstrates consistent labeling within the C. elegans pharynx, with a particular emphasis on the terminal pharyngeal bulb. A magnified image at 40 × magnification is presented, captured using a CY5 filter. The symbol “*” is used to represent a significant difference compared to the control group at a significance level of P < 0.05, while the symbol “#” indicates a significant difference compared to vanadium + ZA-II-05 (V + ZA) group at at the same significance level. Vanadium (100 mM) and ZA-II-05 (1 mg/ml). CON, control; VAN, vanadium; ZA-II-05; ZA