Fig. 4

Co-immunostaining for DPEP1 (green) and TNF-α (red) in control and OxPt-treated rat DRG. Control: low TNF-α fluorescence signal as spots in cytosol of neurons (yellow stars), axon fibres and surrounding cells (red arrowheads), few spots can be found in cells bordering neurons (white arrows). OxPt-treated DRG neurons show increased fluorescence signal for TNF-α (red), in cytosol, varying from low to high. Increased levels of TNF-α-specific fluorescence in glia cells (yellow arrowheads). Some vacuoles or vacuole-like structures in cells of OxPt treated rats. Zeiss LSM980 Airyscan-2 confocal microscope. Bar = 40 μm