The angiotensin II receptors type 1 and 2 modulate astrocytes and their crosstalk with microglia and neurons in an in vitro model of ischemic stroke

Table 1 Primers and parameters of RT-qPCR

RNA	Sequences forward/reverse 5′–3′	Temperature (°C) step 1/2/3	Duration (s) step 1/2/3	Accession number
AT1a	AGTCCTGTTCCACCCGATCA/ CTTTCTGGGAGGGTTGTGTGA	95/56.0/72	15/15/45	NM_030985.4
AT1b	TCAAGCTGTTCTGTCCCA/ GCTACAACTTCAATCAAACAACAGC	95/56.0/72	15/15/45	NM_031009.2
AT2	ACTGGCTCTTTGGACCTGTG/ GCTTGCCAGGGATTCCTTCT	95/56.0/72	15/15/45	NM_012494.3
S100A10	CACACCTTGATGCGTCCTCT/ GGCAACCGGATGCAAACAAT	95/60.0/72	15/15/45	NM_031114.1
Ki67	TCTTGGCACTCACAGTCCAG/ GCTGGAAGCAAGTGAAGTCC	95/58.0/72	15/15/45	NM_001271366.1
EAAT2	GAGAGAGGCTGCCCGTTAAA/ CTTCCACCTGCTTGGGCATA	95/58.0/72	15/15/45	NM_017215.2
iNOS	GCTTGTCTCTGGGTCCTCTG/ CTCACTGGGACAGCACAGAA	95/59.0/72	15/15/45	NM_012611.3
CD206	AACAAGAATGGTGGGCAGTC/ CCTTTCAGTCCTTTGCAAGC	95/56.0/72	15/15/45	NM_001106123.2
IL-6	CCCAACTTCCAATGCTCTCCT/ AGCACACTAGGTTTGCCGAG	95/57.3/72	15/15/45
IL-10	GAAAAATTGAACCACCCGGCA/ TTTCCAAGGAGTTGCTCCCG	95/56/72	15/15/45
Cx43	CTCACGTCCCACGGAGAAAA/ CGCGATCCTTAACGCCTTTG	95/56.0/72	15/15/45	NM_012567.2
RPL13a	TCTCCGAAAGCGGATGAACA/ CAACACCTTGAGGCGTTCCA		15/15/45	NM_173340.2

ISSN: 1471-2202