Fig. 4

Exercise training and/or postbiotic treatment alter mitochondrial composition. A Mitochondrial LONP1, SDHA and NDUFV1 levels were determined by Western blotting in the mitochondrial fraction of the brain of wild type (Wt), control (APP/PS1^TG), trained (APP/PS1^TG-Ex), postbiotic supplemented (APP/PS1^TG-Pt) and combined (trained and postbiotic-supplemented) (APP/PS1^TG-Ex-Pt) mice. Quantitative analysis of optical densities of protein bands for B, C NDUFV1, B, D SDHA and B, E LONP1 expression. Data were normalized to total protein levels determined by Coomassie staining of proteins transferred to the membrane. Bands are elements of the same representative gel, the continuity of the gel was broken, and the irrelevant group was omitted, indicated by white area. Western blot results show means ± SEM (n = 4–5). ^#P < 0.05 compared with APP/PS1 control group vs wild type group, determined by analysis of variance using unpaired Student T-test. *P < 0.05 compared with APP/PS1 groups, determined by analysis of variance using Two-way ANOVA, followed by Bonferroni’s post-hoc test