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Fig. 5 | BMC Neuroscience

Fig. 5

From: Long-term exercise training down-regulates m6A RNA demethylase FTO expression in the hippocampus and hypothalamus: an effective intervention for epigenetic modification

Fig. 5

Exercise increased the level of m6A and down-regulated FTO expression in hippocampus and hypothalamus. a The level of m6A was detected using the ELISA method. n = 6 per group, *p < 0.05. b The heatmaps showed 13 m6A RNA methylation regulators in hippocampus and hypothalamus of the exercise group compared to the sedentary group. hip.E and hyp.E were marked with blue, hip.S and hyp.S were marked with red, position of white spots on the way represented the median value of expression, n = 5 per group, p < 0.05. c qPCR showed 13 m6A RNA methylation regulators mRNA expression. The relative levels of these genes were normalized to β-actin, and the relative mRNA levels in the sedentary group were normalized as “1”. n = 6 per group, ***p < 0.01, ***p < 0.001. d Western blot analysis of FTO protein levels. The relative levels of FTO were normalized to β-actin, and the relative protein levels in the sedentary group were normalized as “1”. n = 6 per group, ***p < 0.001

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BMC Neuroscience

ISSN: 1471-2202

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