Fig. 7

Immunolocalization of cystathionin-β-synthase enzyme (CBS, red) in a–e TRPV1-, f–j TRPA1- and k–o TRPC-positive dorsal root ganglion neurons of diabetic rats (green). a, f and k show a representative 16 µm-slice from dorsal root ganglia. b, g and l show a representative CBS staining with Cy3 from dorsal root neurons, whereas c, h and m show TRPV1, TRPA1 and TRPC6 representative staining with Cy2 in dorsal root neurons, respectively. d, i and n show the merged image from Cy3 and Cy2 signals, the overlapping between CBS and TRP channels is shown in a yellow-orange color. e, j and o show a magnification of d, i and n, respectively