Fig. 1

Photothrombotic model of focal ischemic stroke. a Schematic of the design of laser speckle imaging system (left panel) and a typical LSI image representing CBF information in pseudo-color. Dashed circle indicates the focus of the 532 nm light illumination applied in modeling of focal ischemia. LD laser diode, LSI laser speckle imaging. Scale bar 1 mm. b Schematic of the experimental protocols. ISO isoflurane, MRI magnetic resonance imaging, TTC 2,3,5-triphenyltetrazolium chloride