Figure 4

Distribution of Cln8 mRNA in brains of hippocampal kindling induced epileptic mice. Frontal mouse brain sections were analyzed 2 h, 6 h and 24 h after kindling induced epileptic seizures by in situ hybridization. Mice with electrodes implanted but without electrical stimulations were used as controls (0 h). Dark-field emulsion autoradiographs of hippocampal Cln8 expression in kindling-induced mice 0 h and 24 h after kindling are shown. Optical density values of hybridization signals of Cln8 antisense probe are shown as bars in the diagram. Optical density values of hybridization signals of Cln8 sense probe are shown as a gray area behind the bars. In each brain region the optical density values 2 h, 6 h and 24 h after kidling-induced seizures were compared to controls (0 h). Error bars represent standard error of the mean. Symbols *, ** and *** represent p < 0.05, 0.01 and 0.001, respectively. DG = dentate gyrus