Figure 2

In situ hybridization analysis of Cln8 mRNA expression in mouse embryos. In the E17 mouse embryo, developing gastrointestinal tract (A, B) and DRG (C, D), hybridized with Cln8 specific cRNA probe, are shown. Bright-field view of the cells is shown on the left (A, C) and dark-field detection of hybridization signals on the right (B,D). Optical density values of hybridization signals of Cln8 sense and antisense probes in E13 and E17 gut and brain tissues are shown in the diagram (E). Error bars represent standard error of the mean. s = sense, as = antisense