| | PSC | PSD | PSR |
|---|
W | 53.48 ± 3.65 | 104.55 ± 10.14¶¶¶
| 34.48 ± 4.80###
|
SWS | 72.08 ± 3.93 | 45.63 ± 10.16¶
| 49.40 ± 4.95 * |
PS | 24.6 ± 0.4 | 0.0 ± 0.0¶¶¶
| 65.8 ± 4.0 ***, ###
|
Fos+ | 25.00 ± 11.16 | 160.42 ± 32.13 | 445.24 ± 70.11 *** , ##
|
Hypocretin+ | 92.25 ± 7.40 | 113.07 ± 9.06 | 96.34 ± 3.25 |
Hypocretin+/Fos+ | 0.06 ± 0.06 | 3.31 ± 0.90¶
| 2.03 ± 0.86 |
MCH+ | 201.17 ± 12.18 | 186.50 ± 12.15 | 195.00 ± 8.75 |
MCH+/Fos+ | 0.13 ± 0.13 | 0.27 ± 0.11 | 113.46 ± 4.72 ***, ###
|
- Time spent in W, SWS and PS is reported in minutes, scored per 10 sec epochs over the last 150 minutes of the experiment. The number (mean ± SEM) of Fos+, MCH+, Hypocretin+ and double-labeled neurons (Hypocretin+/Fos+ or MCH+/Fos+) was calculated in the posterior hypothalamus for control (PSC, n = 4), PS deprived (PSD, n = 4) and PS recovery (PSC, n = 4) rats. Cells were counted on 4 and 6 hemi-sections per animal for Hypocretin/Fos and MCH/Fos labelings respectively. Significance values indicated for individual points are: ¶ p < 0.05 and ¶¶¶ p < 0.001 between PSD and PSC, * p < 0.05 and *** p < 0.001 between PSR and PSC, ## p < 0.01 and ### p < 0.001 between PSR and PSD conditions.
